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Apocalypse23.
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http://cellbank.nibio.go.jp/cellbank/quali...l/OL7-11-07.pdf
THE CATHOLIC UNIVERSITY OF AMERICA
The Discovery Center for Cell and Molecular Biology
McCort-Ward Building, Cardinal Station
Washington, DC 20064
An Open Letter Regarding the Misidentification and Cross-Contamination of Cell Lines: Significance and
Recommendations for Correction
Dear Secretary Leavitt:
This letter has been drafted by a group of leading cell culture scientists and other experts involved in the use of DNA methods for the identification of biological material. This group is seriously concerned about the continued use of misidentified and cross-contaminated cell lines in current biomedical research. We urge you to read this letter, share our concerns and take appropriate action consistent with the responsibility of your office.
.....The resulting corruption of biomedical research, no matter the frequency of occurrence, has the potential for grave public health consequences. Drugs, vaccines, and other biomedical products may be developed using misleading or
false data, causing delays in availability of needed medical treatments. Additionally, huge amounts of resources , both public and private, are wasted. Critical time and vast sums of money are wasted in support of research using misidentified or cross-contaminated cell lines and in the development, testing and manufacture of biomedical
products based upon erroneous data. Two real-life examples include:
· An NCI-60, multiple drug-resistant breast cancer cell line has been distributed by the National Cancer Institute (NCI) and widely used to compare other breast cancer cells . The drug-resistant breast cancer cells
were ultimately determined to be ovarian cancer cells .2,3 The discovery authors, Liscovitch and Rabin,
estimate that there are probably 300 published papers with the erroneous identification.
Page 2
· In human metastases studies, human tumor cells placed in animals frequently turn out to be (nonhuman)
mouse cells or recruited host cells .4,5
The irony of the situation is that techniques used for authentication today are inexpensive and fast,6,7 ,8,9 while
the errors caused by lack of cell line authentication are in many cases too costly for society! .........****read all click on link above..
XMRV. Xenotropic/Ecotropic Murine Leukemia Virus ricerca e considerazioni |